7LO1

FAD-dependent monooxygenase AfoD from A. nidulans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	83.542, 96.969, 116.341
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.750 - 2.090
R-factor	0.1891
Rwork	0.187
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nes
RMSD bond length	0.011
RMSD bond angle	1.334
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.750	2.090
High resolution limit [Å]	2.017	2.017
Rmerge	0.119	1.800
Number of reflections	763820	71983
<I/σ(I)>	13.9	1.6
Completeness [%]	99.8	98.3
Redundancy	13.5	13
CC(1/2)	0.997	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	(0.2 M (NH4)2SO4, 0.1 M MES:NaOH pH 6.5, 30% w/v PEG 5K MME).