7LNW
I146A mutant of the isopentenyl phosphate kinase from Candidatus methanomethylophilus alvus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-25 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.249, 92.998, 52.145 |
| Unit cell angles | 90.00, 92.02, 90.00 |
Refinement procedure
| Resolution | 28.680 - 2.290 |
| R-factor | 0.2091 |
| Rwork | 0.204 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Solved Native |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.607 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmeas | 0.155 | 0.584 |
| Rpim | 0.074 | 0.317 |
| Number of reflections | 21038 | 1051 |
| <I/σ(I)> | 8.86 | 1.67 |
| Completeness [%] | 99.9 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 0.1 M Bis-Tris pH 5.5 28% w/v PEG 3350, 0.1 M Sodium chloride |
