7LNJ

CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	82.253, 161.361, 230.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.350 - 2.680
R-factor	0.2123
Rwork	0.210
R-free	0.25070
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7lni
RMSD bond length	0.002
RMSD bond angle	0.431
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.350	2.790
High resolution limit [Å]	2.680	2.680
Rmerge	0.224	2.040
Rpim	0.068	0.690
Number of reflections	75973	6312
<I/σ(I)>	16.3	0.9
Completeness [%]	87.8	73.8
Redundancy	10.3	7
CC(1/2)		0.384

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	292	0.1 M Bis-Tris pH 6.7, 23% polyethylene glycol (PEG) 3350, 0.3 M potassium citrate