7LNG

TNA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	99
Detector technology	PIXEL
Collection date	2021-02-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033175
Spacegroup name	P 3 2 1
Unit cell lengths	45.165, 45.165, 82.831
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	82.830 - 2.200
Rwork	0.225
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uee
RMSD bond length	0.017
RMSD bond angle	2.663
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.830	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.103	0.295
Rmeas	0.111	0.312
Rpim	0.040	0.101
Number of reflections	5325	266
<I/σ(I)>	20	1.3
Completeness [%]	99.7	100
Redundancy	8.9
CC(1/2)	0.996	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES sodium pH 7.5, 1.5 M Lithium sulfate monohydrate