7LNF

3'-deoxy modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2020-12-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 3 2 1
Unit cell lengths	46.822, 46.822, 83.199
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.748 - 1.652
Rwork	0.208
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uee
RMSD bond length	0.025
RMSD bond angle	2.876
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.084	0.393
Rmeas	0.090	0.418
Rpim	0.030	0.142
Number of reflections	13195	658
<I/σ(I)>	23.1	4.5
Completeness [%]	99.8	99.4
Redundancy	10	8
CC(1/2)	1.000	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.02 M Magnesium sulfate hydrate, 0.002 M Cobalt (II) chloride hexahydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0, 25% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.0005 M Spermine