7LMW
Receptor for Advanced Glycation End Products VC1 domain in complex with 3-(3-((4-(4-carboxyphenoxy)benzyl)oxy)phenyl)-1H-indole-2-carboxylic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-08 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0781 |
Spacegroup name | P 62 |
Unit cell lengths | 101.885, 101.885, 102.306 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.410 - 2.500 |
R-factor | 0.2031 |
Rwork | 0.200 |
R-free | 0.25610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lp4 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.411 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.820 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmeas | 0.086 | 0.461 |
Rpim | 0.037 | 0.199 |
Number of reflections | 20999 | 6412 |
<I/σ(I)> | 13 | 2.6 |
Completeness [%] | 99.8 | 100 |
Redundancy | 2.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.5 M NaOAc (pH 7.5) |