7LMN

Structure of full-length human lambda-6A light chain JTO in complex with stabilizer 26 [2-(7-(diethylamino)-4-methyl-2-oxo-2H-chromen-3-yl)ethyl (3-(1H-imidazol-4-yl)benzyl)carbamate]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976484
Spacegroup name	P 21 21 21
Unit cell lengths	64.028, 82.131, 96.451
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.780 - 2.010
R-factor	0.19
Rwork	0.187
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mg5
RMSD bond length	0.009
RMSD bond angle	1.588
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.780	2.120
High resolution limit [Å]	2.010	2.010
Rpim	0.033	0.273
Number of reflections	34240	4798
<I/σ(I)>	14.1	2.8
Completeness [%]	99.6	97.2
Redundancy	7.8	7.8
CC(1/2)	1.000	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	20% PEG 3350 and 0.25 M NH4H2PO4 at 23 degrees C