7LMK

Crystal structure of bovine DNMT1 BAH1 domain in complex with H4K20me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	71.060, 81.372, 129.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.060 - 2.647
R-factor	0.2106
Rwork	0.206
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wxx
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.370	2.780
High resolution limit [Å]	2.647	2.650
Number of reflections	22223	2882
<I/σ(I)>	15.7
Completeness [%]	98.8
Redundancy	4.4
CC(1/2)	0.998	0.404

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	13% PEG1500, 20 mM DTT and 200 mM L-proline, 0.1 M HEPES (pH 7.5)