7LLJ
Inhibitory immune receptor protein complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95373 |
Spacegroup name | H 3 |
Unit cell lengths | 297.549, 297.549, 120.371 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.710 - 3.150 |
R-factor | 0.248 |
Rwork | 0.240 |
R-free | 0.27610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gup 1tvd 4lfh 5d5m |
RMSD bond length | 0.002 |
RMSD bond angle | 0.532 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.890 | 3.220 |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 68693 | 4609 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 100.0 | |
Redundancy | 9.4 | |
CC(1/2) | 0.997 | 0.713 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30-40% PEG 400, 0.1/0.2 HEPES pH 7.5, 0.2M MgCl2 |