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7LLJ

Inhibitory immune receptor protein complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron siteAustralian Synchrotron
BeamlineMX2
Temperature [K]100
Detector technologyPIXEL
Collection date2020-06-24
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.95373
Spacegroup nameH 3
Unit cell lengths297.549, 297.549, 120.371
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution75.710 - 3.150
R-factor0.248
Rwork0.240
R-free0.27610
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4gup 1tvd 4lfh 5d5m
RMSD bond length0.002
RMSD bond angle0.532
Data reduction softwareMOSFLM
Data scaling softwareAimless
Phasing softwarePHENIX
Refinement softwarePHENIX (1.19.1_4122)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]85.8903.220
High resolution limit [Å]3.1503.150
Number of reflections686934609
<I/σ(I)>9.6
Completeness [%]100.0
Redundancy9.4
CC(1/2)0.9970.713
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.529330-40% PEG 400, 0.1/0.2 HEPES pH 7.5, 0.2M MgCl2

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