7LLJ

Inhibitory immune receptor protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	H 3
Unit cell lengths	297.549, 297.549, 120.371
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	75.710 - 3.150
R-factor	0.248
Rwork	0.240
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gup 1tvd 4lfh 5d5m
RMSD bond length	0.002
RMSD bond angle	0.532
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.890	3.220
High resolution limit [Å]	3.150	3.150
Number of reflections	68693	4609
<I/σ(I)>	9.6
Completeness [%]	100.0
Redundancy	9.4
CC(1/2)	0.997	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	30-40% PEG 400, 0.1/0.2 HEPES pH 7.5, 0.2M MgCl2