7LKO
The PilB(N-terminal)-PilZ complex of the Type IV pilus from Xanthomonas citri (2.9 A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-01-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.353, 123.547, 63.539 |
| Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
| Resolution | 44.010 - 2.880 |
| R-factor | 0.1616 |
| Rwork | 0.158 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PilB-PilZ SeMet model |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.454 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.010 | 2.980 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.060 | 0.190 |
| Number of reflections | 13503 | 2614 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 4 mg/ml protein, 0.1 M Tris pH 8.5, 2.0 M ammonium sulfate |
