7LKM
The PilB(N-terminal_P70S mutant)-PilZ complex of the Type IV pilus from Xanthomonas citri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.45866 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.063, 122.408, 62.730 |
Unit cell angles | 90.00, 98.98, 90.00 |
Refinement procedure
Resolution | 28.000 - 2.000 |
R-factor | 0.1815 |
Rwork | 0.179 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PilB-PilZ_SeMet_Model |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 3.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.150 | 1.250 |
Number of reflections | 44979 | 4457 |
<I/σ(I)> | 10.1 | |
Completeness [%] | 99.6 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M Tris pH 8.5; 2.0 M ammonium sulfate |