7LJA

Human TRAAK K+ channel FHEIG mutant A198E in a Tl+ bound conductive conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	PIXEL
Collection date	2019-10-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9778
Spacegroup name	P 1 21 1
Unit cell lengths	80.230, 136.530, 95.280
Unit cell angles	90.00, 94.54, 90.00

Refinement procedure

Resolution	47.540 - 2.770
R-factor	0.2034
Rwork	0.201
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lj5
RMSD bond length	0.010
RMSD bond angle	1.590
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.540	3.021
High resolution limit [Å]	2.770	2.770
Rmerge		3.376
Rmeas		3.438
Rpim		0.648
Number of reflections	35508	2217
<I/σ(I)>	9.8	1.338
Completeness [%]	93.4	19.56
Redundancy	27.7	14.03
CC(1/2)	0.995	0.581

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	277	50mM Tris pH 8.8, 64-200 mM CaCl2, 27-33% (vol/vol) PEG400