7LIR

Structure of the invertebrate ALK GRD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	I 41 2 2
Unit cell lengths	224.339, 224.339, 114.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.180 - 2.600
R-factor	0.2255
Rwork	0.223
R-free	0.27010
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.594
Data reduction software	XDS (20200417)
Data scaling software	SCALA (0.7.4)
Phasing software	SHELX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	112.420	50.180	2.700
High resolution limit [Å]	2.600	9.720	2.600
Rmerge	0.205	0.058	8.318
Rmeas	0.209	0.060	8.479
Rpim	0.040	0.012	1.632
Total number of observations		22494	122935
Number of reflections	44990	959	4617
<I/σ(I)>	14.9	58.5	0.5
Completeness [%]	99.9	99.3	99.3
Redundancy	26.9	23.5	26.6
CC(1/2)	0.999	0.999	0.425

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	289.15	1.84 M (NH4)2SO4, Bicine pH 9.0, 28 mg/ml protein