7LIP

X-ray structure of SPOP MATH domain (D140G)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-15
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	84.766, 43.450, 51.026
Unit cell angles	90.00, 123.22, 90.00

Refinement procedure

Resolution	21.320 - 1.480
R-factor	0.1823
Rwork	0.180
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lin
RMSD bond length	0.008
RMSD bond angle	0.977
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.16_3549)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.320	1.530
High resolution limit [Å]	1.480	1.480
Rmerge	0.085	0.660
Rmeas	0.091	0.720
Rpim	0.032	0.260
Number of reflections	25043	2391
<I/σ(I)>	20.56	3.24
Completeness [%]	95.9
Redundancy	8.1
CC(1/2)	1.000	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Reservoir solution: 0.1 M sodium citrate tribasic dihydrate, pH 5.6, 0.2 M (NH4)2SO4, 1 M Li2SO4. Two ul of the protein at 24 mg/ml in 20 mM Tris-HCl, pH 7.6, 150 mM NaCl, 5 mM DTT was mixed with 2 ul of the reservoir solution. The reservoir solution was 0.5 ml.