7LHX

Human U1A protein with F37M and F77M mutations for improved phasing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS MICROFOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-12
Detector	APEX II CCD
Wavelength(s)	1.5418
Spacegroup name	P 3 2 1
Unit cell lengths	64.377, 64.377, 46.828
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.880 - 2.200
R-factor	0.1681
Rwork	0.166
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1urn
Data reduction software	PROTEUM PLUS (2019.11-0)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.880	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.029	0.076
Rpim	0.009	0.038
Number of reflections	10961	558
<I/σ(I)>	61.2	20.2
Completeness [%]	100.0	100
Redundancy	10	4.8
CC(1/2)	1.000	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	2.7-3.1 M Sodium Acetate