7LHX
Human U1A protein with F37M and F77M mutations for improved phasing
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER IMUS MICROFOCUS |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-12 |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 64.377, 64.377, 46.828 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.880 - 2.200 |
R-factor | 0.1681 |
Rwork | 0.166 |
R-free | 0.21280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1urn |
Data reduction software | PROTEUM PLUS (2019.11-0) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.880 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.029 | 0.076 |
Rpim | 0.009 | 0.038 |
Number of reflections | 10961 | 558 |
<I/σ(I)> | 61.2 | 20.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10 | 4.8 |
CC(1/2) | 1.000 | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 2.7-3.1 M Sodium Acetate |