7LHA

Structure of the Exo-L-galactose-6-sulfatase BuS1_11 from Bacteroides uniformis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-14
Detector	RAYONIX MX-300
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	89.410, 89.410, 264.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.600 - 1.950
R-factor	0.1505
Rwork	0.148
R-free	0.19350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qzu
RMSD bond length	0.009
RMSD bond angle	0.949
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.990
High resolution limit [Å]	1.950	1.950
Rmerge	0.166	0.920
Rpim	0.053	0.178
Number of reflections	79225	79363
<I/σ(I)>	13.8	2.3
Completeness [%]	100.0	100
Redundancy	10.5	10.4
CC(1/2)	0.993	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M HEPES (pH 7.5), 0.2 M MgCl2, 25% (w/v) PEG 3350