7LH6

The structure of Bacteroides plebeius L-galactose dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-21
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	55.754, 84.461, 129.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.870 - 2.850
R-factor	0.2371
Rwork	0.232
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ynp
RMSD bond length	0.003
RMSD bond angle	0.714
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.106	0.819
Rpim	0.046	0.478
Number of reflections	14732	704
<I/σ(I)>	14.7	1.7
Completeness [%]	98.6	95.5
Redundancy	5.8	3.5
CC(1/2)	0.994	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.2 M ammonium citrate dibasic (pH 5.5), 20% (w/v) PEG 3350