7LGN

Cyanophycin synthetase 1 from T. morbirosei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	I 41
Unit cell lengths	222.561, 222.561, 105.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.310 - 3.100
Rwork	0.270
R-free	0.32600
Structure solution method	SAD
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.310	3.211
High resolution limit [Å]	3.100	3.100
Rmerge	0.067	0.404
Rmeas	0.095	0.572
Rpim	0.067	0.404
Number of reflections	46806	4632
<I/σ(I)>	11.47	0.8
Completeness [%]	99.9	99.42
Redundancy	2	2
CC(1/2)	0.995	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.8	277	13.25% PEG3350, 320mM NaFormate, 1% glycerol, 100mM Na/K phosphate pH6.8. protein concentration 6mg/ml.