7LFQ
Pyrococcus RNA ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 151.379, 151.379, 82.337 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.870 - 2.700 |
| R-factor | 0.1931 |
| Rwork | 0.191 |
| R-free | 0.21610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dwr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.351 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874+SVN) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
| Rmerge | 0.084 | 0.024 | 1.311 |
| Rmeas | 0.088 | 0.025 | 1.375 |
| Rpim | 0.026 | 0.008 | 0.411 |
| Total number of observations | 293064 | ||
| Number of reflections | 26802 | 1502 | 1311 |
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 10.9 | 10.8 | 11.1 |
| CC(1/2) | 1.000 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | Morpheus G6 |
