7LFN

Structure of Hyperglycosylated Human IgG1 Fc (Fc267_329)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.033190
Spacegroup name	P 21 21 21
Unit cell lengths	49.483, 79.253, 141.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.750 - 2.600
R-factor	0.2318
Rwork	0.230
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jii
RMSD bond length	0.010
RMSD bond angle	1.052
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.750	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.044
Rpim	0.042	0.272
Number of reflections	17601	2103
<I/σ(I)>	11.8	2.6
Completeness [%]	99.2
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	CRYSTALS GROWN BY MIXING 1 UL OF FC267_329 (10 MG/ML IN 10mM HEPES, 75mM NaCl pH 7.4) WITH 1 UL OF PRECIPITANT SOLUTION CONSISTING OF 0.025M MES pH 7.0, 0.1M KCl, 20.571% w/v PEG3350