7LFF
Crystal structure of the Candida albicans kinesin-8 motor domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.990, 52.680, 86.750 |
| Unit cell angles | 86.90, 80.00, 89.92 |
Refinement procedure
| Resolution | 45.910 - 2.010 |
| R-factor | 0.2341 |
| Rwork | 0.232 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lre |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.047 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.910 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.114 | |
| Rmeas | 0.141 | |
| Rpim | 0.082 | |
| Number of reflections | 49650 | 19655 |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 97.4 | 96.7 |
| Redundancy | 2.7 | |
| CC(1/2) | 0.149 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M MMT, 25% PEG 1500, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
