7LFF

Crystal structure of the Candida albicans kinesin-8 motor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	43.990, 52.680, 86.750
Unit cell angles	86.90, 80.00, 89.92

Refinement procedure

Resolution	45.910 - 2.010
R-factor	0.2341
Rwork	0.232
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lre
RMSD bond length	0.005
RMSD bond angle	1.047
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.910	2.080
High resolution limit [Å]	2.010	2.010
Rmerge	0.114
Rmeas	0.141
Rpim	0.082
Number of reflections	49650	19655
<I/σ(I)>	4.6
Completeness [%]	97.4	96.7
Redundancy	2.7
CC(1/2)		0.149

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.1 M MMT, 25% PEG 1500, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K