7LF9

Structure of Hyperglycosylated Human IgG1 Fc (Fc329)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.033190
Spacegroup name	P 21 21 21
Unit cell lengths	49.478, 77.964, 140.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.640 - 2.200
R-factor	0.2251
Rwork	0.224
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cdh
RMSD bond length	0.010
RMSD bond angle	1.123
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.640	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.037
Rpim	0.028	0.028
Number of reflections	28367	2429
<I/σ(I)>	15.5	2.8
Completeness [%]	99.9
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	CRYSTALS GROWN BY MIXING 1 UL OF FC329 (10 MG/ML IN 10mM HEPES, 75mM NaCl pH 7.4) WITH 1 UL OF PRECIPITANT SOLUTION CONSISTING OF 0.1M HEPES pH 6.5, 12.571% w/v PEG6000