7LEU

Structure of importin a2 bound to p65-NLS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	78.556, 90.857, 99.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.820
R-factor	0.2025
Rwork	0.201
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y2a
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.910
High resolution limit [Å]	2.810	2.810
Rpim	0.073	0.730
Number of reflections	17271	1551
<I/σ(I)>	14.5	1.5
Completeness [%]	97.7	89.3
Redundancy	6.9	4.6
CC(1/2)	0.995	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.55M Na citrate, 100mM HEPES pH 6.0