7LDK
Structure of human respiratory syncytial virus nonstructural protein 2 (NS2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 62 |
| Unit cell lengths | 157.646, 157.646, 47.291 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.550 - 2.820 |
| R-factor | 0.21394 |
| Rwork | 0.212 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in-house Se-Met-labeled NS2 model solved by SAD phasing |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.589 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 2.154 |
| Rmeas | 0.090 | 2.154 |
| Rpim | 0.033 | 0.842 |
| Number of reflections | 16685 | 740 |
| <I/σ(I)> | 24.8 | 0.82 |
| Completeness [%] | 99.4 | 91.1 |
| Redundancy | 7.1 | 5.9 |
| CC(1/2) | 0.995 | 0.432 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.4 M potassium-sodium-tartrate |
