7LDG

Crystal structure of the MEILB2-BRCA2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-10
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.97895
Spacegroup name	H 3 2
Unit cell lengths	179.889, 179.889, 179.186
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.100 - 2.560
Rwork	0.211
R-free	0.23070
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.611
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1-3600)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.940	2.700
High resolution limit [Å]	2.560	2.560
Rmerge	0.088	1.467
Number of reflections	35987	5188
<I/σ(I)>	18.9	2
Completeness [%]	100.0	100
Redundancy	21	21.7
CC(1/2)	0.999	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	289	0.1 M sodium acetate (pH 4.6), 2 M lithium acetate