7LCY

Crystal structure of the ligand-free ARM domain from Drosophila SARM1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	63.301, 101.363, 98.574
Unit cell angles	90.00, 103.77, 90.00

Refinement procedure

Resolution	47.870 - 3.350
R-factor	0.2458
Rwork	0.244
R-free	0.27170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.437
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.870	3.850
High resolution limit [Å]	3.350	3.350
Number of reflections	17198	3286
<I/σ(I)>	4.7
Completeness [%]	97.7
Redundancy	3.4
CC(1/2)	0.996	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.7 M sodium malonate pH 5.8