7LCV
Factor H enhancing human antibody fragment (Fab) to meningococcal Factor H binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.11 |
Spacegroup name | P 1 |
Unit cell lengths | 39.470, 52.370, 84.560 |
Unit cell angles | 73.78, 84.48, 74.17 |
Refinement procedure
Resolution | 81.180 - 1.700 |
R-factor | 0.2401 |
Rwork | 0.239 |
R-free | 0.26970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3CVD and Fab homology model |
RMSD bond length | 0.017 |
RMSD bond angle | 2.024 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.180 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.052 | 0.836 |
Rmeas | 0.061 | 0.983 |
Rpim | 0.032 | 0.513 |
Number of reflections | 65915 | 6477 |
<I/σ(I)> | 11.59 | 1.3 |
Completeness [%] | 93.1 | 93.1 |
Redundancy | 3.6 | 3.6 |
CC(1/2) | 0.998 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2 M LiNO3, 16% PEG 3350 |