7LCE
Structure of D-Glucosaminate-6-phosphate Ammonia-lyase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 60.374, 171.569, 195.054 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.370 - 2.580 |
R-factor | 0.1941 |
Rwork | 0.193 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lc0 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.516 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.370 | 2.676 |
High resolution limit [Å] | 2.580 | 2.580 |
Number of reflections | 48940 | 8157 |
<I/σ(I)> | 18.1 | |
Completeness [%] | 94.6 | |
Redundancy | 14.6 | |
CC(1/2) | 0.999 | 0.629 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl, pH 8.5, 0.4 M CaCl2, 11% PEG 4000, 8% glycerol |