7LC1
Crystal Structure of KRAS4b (GMPPNP-bound) in complex with the RBD-PH domains of SIN1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.760, 97.070, 96.620 |
Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
Resolution | 48.540 - 2.350 |
R-factor | 0.2344 |
Rwork | 0.231 |
R-free | 0.27920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3voq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.540 | 48.540 | 2.490 |
High resolution limit [Å] | 2.350 | 10.300 | 2.350 |
Rmerge | 0.074 | 0.032 | 0.853 |
Rmeas | 0.088 | 0.038 | 1.012 |
Total number of observations | 109727 | ||
Number of reflections | 32468 | 408 | 5198 |
<I/σ(I)> | 10.16 | 27.4 | 1.81 |
Completeness [%] | 98.6 | 98.8 | 99.3 |
Redundancy | 3.38 | 3.311 | 3.437 |
CC(1/2) | 0.997 | 0.998 | 0.678 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 200 mM ammonium sulfate, 100 mM HEPES (N-2-hydroxyethyl piperazine-N-ethane sulfonic acid) pH 7.5, 25% PEG 3350 |