7LBX
Crystal structure of TFAM (mitochondrial transcription factor A) in complex with LSP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.425, 120.507, 55.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.120 - 2.700 |
| R-factor | 0.2099 |
| Rwork | 0.208 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tq6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.347 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.600 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.046 | 0.884 |
| Number of reflections | 21463 | 987 |
| <I/σ(I)> | 29.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1M HEPES (pH 7.5), 0.05 M MgCl2, and 32.5% PEG MME 550 |
