7LBK
Crystal structure of human Survivin bound to histone H3 T3phK4me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-21 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.27822 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.292, 71.369, 82.554 |
| Unit cell angles | 90.00, 129.33, 90.00 |
Refinement procedure
| Resolution | 41.040 - 2.700 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.699 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.094 | 0.756 |
| Number of reflections | 13890 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 98.1 | 93 |
| Redundancy | 14.8 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | The crystal was obtained by mixing 1 uL of protein at 10 mg/mL with 1 uL of mother liquor composed of 0.16 M potassium/sodium tartrate, 14% PEG 3350. |
