7LB0
Crystal structure of the tandem bromodomain (BD1, BD2) of human TAF1 bound to ZS1-295
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-08-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.258, 54.735, 122.968 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.480 - 2.330 |
| R-factor | 0.1985 |
| Rwork | 0.197 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7k27 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4085) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 61.480 | 61.480 | 2.410 |
| High resolution limit [Å] | 2.330 | 9.020 | 2.330 |
| Rmerge | 0.299 | 0.038 | 1.511 |
| Rmeas | 0.322 | 0.042 | 1.627 |
| Rpim | 0.119 | 0.017 | 0.598 |
| Total number of observations | 1663 | 9642 | |
| Number of reflections | 25218 | 287 | 1320 |
| <I/σ(I)> | 7.5 | 28.9 | 1.4 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 7.2 | 5.8 | 7.3 |
| CC(1/2) | 0.987 | 1.000 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M MgCl2, 0.1 M Bis-Tris pH 6.5, 25% PEG 3350 |
