7LA7

O6 variable lymphocyte receptor ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	52.450, 38.276, 78.690
Unit cell angles	90.00, 103.79, 90.00

Refinement procedure

Resolution	27.099 - 1.551
R-factor	0.175958079504
Rwork	0.174
R-free	0.21484
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g39
RMSD bond length	0.011
RMSD bond angle	1.086
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11_2567)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.100	1.610
High resolution limit [Å]	1.550	1.550
Rpim	0.044	0.501
Number of reflections	125962	2093
<I/σ(I)>	12.7	1.2
Completeness [%]	99.6
Redundancy	5.7
CC(1/2)	0.894	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	293.15	1.2 M NaH2PO4, 0.8 M K2HPO4, 0.1 M glycine pH 10.5, 0.2 M Li2SO4