7L84

Hen Egg White Lysozyme by Native S-SAD at Room Temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	295
Detector technology	PIXEL
Collection date	2019-07-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.892
Spacegroup name	P 43 21 2
Unit cell lengths	79.344, 79.344, 37.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.100 - 1.700
R-factor	0.1409
Rwork	0.140
R-free	0.16210
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.910
Data reduction software	DIALS (3.1.4)
Data scaling software	Aimless (0.7.4)
Phasing software	AutoSol (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.100	1.770
High resolution limit [Å]	1.700	1.700
Rmerge	0.102	0.189
Rmeas	0.103	0.208
Rpim	0.015	0.084
Number of reflections	22855	767
<I/σ(I)>	35.63	6.7
Completeness [%]	90.7	30.45
Redundancy	35.7	5.9
CC(1/2)	0.999	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM citrate buffer (pH 5.2 - 5.8), 20-25% PEG 4000, 20-25% glycerol