7L7V

Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E/R99E/K100E/R103E/K106E/K110E mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03317
Spacegroup name	P 21 2 21
Unit cell lengths	84.332, 89.656, 149.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.610 - 2.950
R-factor	0.2205
Rwork	0.219
R-free	0.24030
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.628
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	3.000
High resolution limit [Å]	2.950	7.990	2.950
Rmerge	0.130	0.045	1.687
Rmeas	0.135	0.047	1.793
Rpim	0.039	0.014	0.594
Total number of observations	289192
Number of reflections	24727	1371	1218
<I/σ(I)>	4.9
Completeness [%]	99.8	99.9	100
Redundancy	11.7	11.4	8.5
CC(1/2)		0.999	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M MES (pH 5.5), 1 M Potassium Sodium Tartrate