7L78

H235C variant of Yeast Ferrochelatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-20
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	49.987, 51.632, 287.336
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.926 - 2.400
R-factor	0.236
Rwork	0.233
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lbq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.520
High resolution limit [Å]	2.400	2.400
Number of reflections	32665	3102
<I/σ(I)>	17.7
Completeness [%]	99.2
Redundancy	3.2
CC(1/2)	0.995	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 2000, 2-propanol, Tris-HCl pH 7.5