7L6J
Crystal Structure of the Putative Hydrolase from Stenotrophomonas maltophilia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-06-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 171.693, 171.693, 171.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.450 - 1.780 |
| R-factor | 0.1382 |
| Rwork | 0.137 |
| R-free | 0.15440 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.376 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.111 | 0.886 |
| Rmeas | 0.117 | 0.931 |
| Rpim | 0.036 | 0.287 |
| Number of reflections | 41390 | 2047 |
| <I/σ(I)> | 23 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.3 | 10.4 |
| CC(1/2) | 0.994 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | Protein: 7.9 mg/ml, 0.5M Sodium chloride, 0.01M Tris pH 8.3; Screen: ComPAS (H12), 3.0M Sodium formate; Cryo: 4.0M Sodium formate. |
