7L6G

MbnP from Methylosinus trichosporium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	77
Detector technology	PIXEL
Collection date	2020-02-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.377
Spacegroup name	P 61
Unit cell lengths	91.276, 91.276, 383.567
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.890 - 2.040
R-factor	0.2067
Rwork	0.204
R-free	0.25100
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	77.450	77.450	2.070
High resolution limit [Å]	2.040	11.170	2.040
Rmerge	0.348	0.086	9.169
Rmeas	0.353	0.087	9.450
Rpim	0.052	0.011	2.241
Total number of observations		45930	83081
Number of reflections	112407	735	4848
<I/σ(I)>	13.91	35.5	1.6
Completeness [%]	98.4	99.8	84.7
Redundancy	50.6	62.5	17.1
CC(1/2)	0.990	0.997	0.379

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M lithium chloride, 1.6 M ammonium sulfate