7L68

C-type carbohydrate-recognition domain 4 of the mannose receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	31.623, 58.718, 62.383
Unit cell angles	90.00, 97.66, 90.00

Refinement procedure

Resolution	31.340 - 1.400
R-factor	0.1558
Rwork	0.155
R-free	0.17490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jub
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.340	31.340	1.420
High resolution limit [Å]	1.400	7.670	1.400
Rmerge	0.057	0.082	0.111
Rmeas	0.061	0.092	0.120
Rpim	0.022	0.038	0.044
Total number of observations	309250	1877	14754
Number of reflections	42022	284	2009
<I/σ(I)>	23.6	32.4	12.4
Completeness [%]	94.4	97.9	92.6
Redundancy	7.4	6.6	7.3
CC(1/2)	0.997	0.972	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 6mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 10mM Man9GlcNAc2. Reservoir solution: 2.5% Peg 8K, 0.1M MES, pH 6.0