7L5A

Crystal structure of the photosensory module from Xanthomonas campestris bacteriophytochrome XccBphP in the Pfr state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	I 41
Unit cell lengths	164.378, 164.378, 40.437
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.980 - 2.950
R-factor	0.189
Rwork	0.185
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5akp
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.980	3.130
High resolution limit [Å]	2.950	2.950
Rmerge	0.122	0.738
Rmeas	0.136	0.818
Rpim	0.058	0.348
Number of reflections	11755	1877
<I/σ(I)>	8.8
Completeness [%]	100.0	100
Redundancy	5.4	5.5
CC(1/2)	0.994	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	294	6% (w/v) polyethylene glycol (PEG) 4000, 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 10% (v/v) isopropanol