7L4V

C-terminal bZIP domain of human C/EBPbeta Bound to DNA with Consensus Recognition with GT Mismatch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	101.452, 113.144, 75.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.770 - 1.750
R-factor	0.2004
Rwork	0.199
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mg2
RMSD bond length	0.005
RMSD bond angle	0.773
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc3_4028)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.770	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.147	1.762
Rpim	0.025	0.496
Number of reflections	44214	4352
<I/σ(I)>	23.8	1.3
Completeness [%]	99.9	99.1
Redundancy	30.6	11.3
CC(1/2)		0.541

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	50 mM ammonium sulfate, 50 mM BIS-TRIS pH 6.5, 30% (v/v) pentaerythritol ethoxylate (15/4 EO/OH)