7L4N

Crystal structure of the DRM2 (C397R)-CCG DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	C 2 2 21
Unit cell lengths	118.292, 231.692, 54.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.202 - 2.247
R-factor	0.1817
Rwork	0.180
R-free	0.20950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4onj
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.247	4.850	2.250
Rmerge	0.155	0.053	1.619
Rmeas	0.166	0.057	1.754
Rpim	0.060	0.020	0.643
Number of reflections	35193	3581	3482
<I/σ(I)>	3.2
Completeness [%]	97.6	93.9	98.4
Redundancy	7.2	7.3	6.8
CC(1/2)		0.998	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	2% v/v Tacsimate(pH 6.0), 0.1 M BIS-TRIS (pH 6.5) and 20% w/v Polyethylene glycol 3,350.