7L4M
Crystal structure of the DRM2-CCT DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9774 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 171.924, 70.981, 104.087 |
Unit cell angles | 90.00, 97.90, 90.00 |
Refinement procedure
Resolution | 47.056 - 2.805 |
R-factor | 0.2168 |
Rwork | 0.213 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4onj |
RMSD bond length | 0.004 |
RMSD bond angle | 0.629 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.098 | 0.049 | 0.998 |
Rmeas | 0.114 | 0.057 | 1.174 |
Rpim | 0.058 | 0.029 | 0.611 |
Total number of observations | 108188 | ||
Number of reflections | 30526 | 3172 | 2943 |
<I/σ(I)> | 7.1 | ||
Completeness [%] | 99.5 | 99.9 | 96.5 |
Redundancy | 3.5 | 3.6 | 3.3 |
CC(1/2) | 0.995 | 0.554 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M Sodium formate pH 7.0, 12% w/v Polyethylene glycol 3,350. |