7L4K

Crystal structure of the DRM2-CCG DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-08-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	C 2 2 21
Unit cell lengths	117.968, 231.582, 54.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.318 - 2.610
R-factor	0.2156
Rwork	0.213
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4onj
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.200	49.200	2.730
High resolution limit [Å]	2.610	9.040	2.610
Rmerge	0.190	0.035	1.639
Rmeas	0.212	0.040	1.860
Rpim	0.091	0.017	0.859
Total number of observations	115890	2743	11827
Number of reflections	23003	596	2753
<I/σ(I)>	7.6	29.7	0.8
Completeness [%]	99.5	95.8	99.3
Redundancy	5	4.6	4.3
CC(1/2)	0.990	0.998	0.409

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	2% v/v Tacsimate(pH 6.0), 0.1 M BIS-TRIS (pH 6.5) and 20% w/v Polyethylene glycol 3,350.