7KZH
Zinc finger antiviral protein (ZAP) central domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 89.637, 89.637, 53.055 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.810 - 2.490 |
R-factor | 0.2279 |
Rwork | 0.226 |
R-free | 0.26310 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.529 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.18_3845) |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.143 | 1.000 |
Rmeas | 0.188 | 1.000 |
Rpim | 0.028 | 0.244 |
Number of reflections | 8779 | 435 |
<I/σ(I)> | 24.2 | 5 |
Completeness [%] | 99.5 | 100 |
Redundancy | 48 | 24 |
CC(1/2) | 0.998 | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.8-1.1 M sodium nitrate 0.1 M sodium acetate |