7KYQ

Crystal structure of human BCCIP beta (Native1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	112.670, 112.670, 56.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.840 - 3.060
R-factor	0.2191
Rwork	0.217
R-free	0.25070
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.539
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.090
High resolution limit [Å]	3.040	3.040
Number of reflections	7295	341
<I/σ(I)>	12.4	2.3
Completeness [%]	99.8
Redundancy	12.4
CC(1/2)	0.995	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M tri-sodium citrate, 18-20% PEG3350