7KWE
Crystal structure of the catalytic domain of human PDE3A bound to DNMDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.976251 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.210, 58.520, 157.540 |
| Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
| Resolution | 47.700 - 2.000 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.21920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1so2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.732 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.057 | 0.747 |
| Number of reflections | 187181 | 29075 |
| <I/σ(I)> | 10.79 | 1.47 |
| Completeness [%] | 94.3 | 91.7 |
| Redundancy | 1.8 | 1.7 |
| CC(1/2) | 0.998 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293.15 | MES, PEG 3350, calcium acetate |
