7KV5
Surface glycan-binding protein B from Bacteroides fluxus
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 59.533, 77.619, 146.621 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.709 - 1.820 |
Rwork | 0.191 |
R-free | 0.23010 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.555 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.710 | 1.860 |
High resolution limit [Å] | 1.820 | 1.820 |
Number of reflections | 30831 | 1532 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 99.7 | |
Redundancy | 6.6 | |
CC(1/2) | 0.999 | 0.934 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 0.1 M MES pH6.6, 0.2 M ammonium sulfate, 20 % (w/v) PEG5000MME 0.1 M guanidine hydrochloride |