7KUO

FANA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2020-06-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977408
Spacegroup name	P 3 2 1
Unit cell lengths	49.072, 49.072, 81.254
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.281
Rwork	0.204
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uee
RMSD bond length	0.027
RMSD bond angle	3.366
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.280	2.280
Rmerge	0.113	0.491
Rmeas	0.117	0.504
Rpim	0.114	0.027
Number of reflections	5536	285
<I/σ(I)>	29.2	7.5
Completeness [%]	100.0	100
Redundancy	18.8	19.2
CC(1/2)	0.999	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2.4 M Sodium malonate pH 7.0, 50 mM Magnesium chloride