7KTM

DNA Polymerase Mu (K438D), 8-oxodGTP:Ct Reaction State Ternary Complex, 50 mM Mn2+ (30min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.241, 62.223, 118.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.980 - 1.528
R-factor	0.1614
Rwork	0.160
R-free	0.18050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.560
High resolution limit [Å]	1.528	4.150	1.530
Rmerge	0.085	0.068	0.949
Rmeas	0.094	0.075	1.032
Rpim	0.038	0.031	0.400
Number of reflections	67772	3580	3344
<I/σ(I)>	12.1
Completeness [%]	99.2	97	99
Redundancy	6.2	5.6	6.4
CC(1/2)		0.993	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000